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Overview
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Human rhinovirus 3C protease (HRV 3C Protease) is a cysteine protease that recognizes the Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro sequence (also called the PreScission site), and cleaves between Gln and Gly. The recombinant GST-HRV 3C can be used to cleave the GST tag in fusion proteins expressed using the pGEX-6p vector or other vectors, then removed by glutathione resin. It exhibits high specificity and activity at 4 0C in an overnight incubation reaction containing 1 mass unit of HRR 3C and 100 mass units of targeting protein.
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Overview