GPCR Signaling Phospho Antibody Array

GPCR Phospho Antibody Array features 193 highly specific antibodies related to G-protein-coupled receptors signaling to MAPK/ERK. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

GPCR Signaling to MAPK/ERK Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features:
· Site-specific phosphorylation profiling and screening with 193 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for analyzing two samples (untreated vs. treated)
· Featured Antibodies: 193 site-specific and phospho-specific antibodies; 6 replicates per antibody
· Reactivity: Human: 100% | Mouse: 95% | Rat: 81%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
Arrestin-1 (Ser412), B-Raf (Ser446), B-Raf (Ser601), B-Raf (Thr598), CaMK2 (Thr286), CaMK2-b/g/d (Thr287), CaMK4 (Thr196/200), CaMK5, CDC25A (Ser124), CDC25A (Ser75), CDC25B (Ser323), CDC25B (Ser353), CDC25C (Ser216), CDC25C (Thr48), c-PLA2 (Ser505), c-Raf (Ser296), c-Raf (Ser43), DYN1 (Ser774), ERK1/2, ERK3 (Ser189), ERK8 (Thr175/Tyr177), FAK (Ser910), FAK (Tyr397), FAK (Tyr407), FAK (Tyr576), FAK (Tyr861), FAK (Tyr925), FOXO1/3/4-PAN (Thr24/32), FOXO1A/3A (Ser322/325), FOXO3A (Ser253), GRB2 (Ser159), GRK2 (Ser29), GRK2 (Ser685), GTPase activating protein (Ser387), Integrin b1 (Thr788), Integrin b3 (Tyr773), Integrin b3 (Tyr785), Integrin b5, JNK1/2/3 (Thr183/Tyr185), KSR (Ser392), KSR2, MAPKAPK2 (Ser272), MAPKAPK2 (Thr222), MAPKAPK2 (Thr334), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), MKP-1 (Ser359), MKP-1/2 (Ser296), MSK1 (Ser212), MSK1 (Ser360), MSK1 (Ser376), MSK1 (Thr581), MSK2 (Thr568), p44/42MAPK (Thr202), p44/42MAPK (Tyr204), P90RSK (Ser380), P90RSK (Thr359/Ser363), P90RSK (Thr573), PEA-15 (Ser104), PEA-15 (Ser116), PI3K-p85-a/g (Tyr467/Tyr199), PKA CAT (Thr197), PKC alpha (Tyr657), PKC alpha/beta II (Thr638), PKC beta (Ser661), PKC delta (Tyr52), PKC delta (Ser645), PKC delta (Thr505), PKC delta (Tyr313), PKC delta (Tyr64), PKC epsilon (Ser729), PKC pan activation site, PKC theta (Ser676), PKC theta (Thr538), PKC zeta (Thr410), PKC zeta (Thr560), PKD1/PKC mu (Ser205), PKD1/PKC mu (Ser910), PKD1/PKC mu (Tyr463), PLC beta (Ser1105), PLC beta3 (Ser537), Pyk2 (Tyr402), Pyk2 (Tyr579), Pyk2 (Tyr580), Pyk2 (Tyr881), RASE, RASF4, Ras-GRF1 (Ser916), RGS16 (Tyr168), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), SYN1 (Ser62), SYN1 (Ser9), TAK1 (Thr184), Tyrosine Hydroxylase (Ser19), Tyrosine Hydroxylase (Ser31), Tyrosine Hydroxylase (Ser40), Tyrosine Hydroxylase (Ser8)

Assay Outline:
The ELISA based GPCR Signaling To MAPK/ERK Phospho Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.