GH Plant Recombinant Protein

GH Plant Recombinant Protein

Catalog Number:
P001417799ABE
Mfr. No.:
Abe32-1260
Price:
  • Size:
    10 µg
    Quantity:
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      • Overview
        • GH human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 205 amino acids (molecular formula C1025H1570N280O306S7) and 6-His-tag at the N-terminal having the total molecular mass of 22.9kDa. GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

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      • Properties
        • Protein Name
          GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin.
          Source
          Nicotiana benthamiana
          Type
          Recombinant Proteins
          Purification
          Greater than 97.0% as determined by Analysis by SDS-PAGE.
          Formulation
          Lyophilized from 1mg/ml solution in PBS 0.05M buffer at pH 7.5.
          Storage
          Lyophilized GH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
          Activity
          Biological Activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 < 0.04-0.1 ng/mL.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized GH in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 < 0.04-0.1 ng/mL.

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