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Overview
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GAS7 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 436 amino acids (1-416a.a.) and having a molecular mass of 49.8 kDa. GAS7 is fused to 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques. GAS7 is mainly expressed in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 is involved in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Over-expression of GAS7 initiats the development of human osteosarcoma.
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Overview