GAPDH Polyclonal Antibody (2mg/mL) for WB, IF

GAPDH Polyclonal Antibody (2mg/mL) for WB, IF

Catalog Number:
A001511357RED
Mfr. No.:
RD86624A
Price:
$205
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      • Overview
        • Calculated MW: 43kDa
          Observed MW: 43kDa

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          Background

          Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

      • Properties
        • Host
          rabbit
          Antibody Type
          polyclonal
          Isotype
          IgG
          Reactivity
          human; mouse; rat; zebrafish
          Immunogen
          Recombinant human GAPDH protein expressed by E.coli
          Purification
          Antigen affinity purification
          Conjugate
          Unconjugated
          Formulation
          PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
          Storage
          Store at -20°C Valid for 12 months. Avoid freeze-thaw cycles.
          Concentration
          2mg/mL
          Antigen
          aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component (Gene ID:317743; UniProt:Q5XJ10)

          * For reference only.

      • Applications
        • Application
          WB; IF
        • Application Dilutions
          WB 1:1000-1:2000
          IF 1:100-1:400

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