FuniCut™ BspQI GMP-grade (10 U/μL)

This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.

Feature

Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GCTCTTC (1/4)
Digestion of DNA to obtain specific sticky ends
Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, RNases

Expression Host: Recombinant E. coli with BspQI gene
Reaction Temperature: 50°C
Storage Buffer: 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol
Unit Definition: 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50°C in a 50 μL system

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