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Overview
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This product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1/N5-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.
This product is produced in accordance with GMP process requirements and provided in a liquid form.
Expression Host: Recombinant E. coli with BasI gene
Reaction Temperature: 37°C
Storage Buffer: 10 mM Tris-HCl, 0.2 M NaCl, 0.1 mM EDTA, 1mM DTT, 50% Glycerol
Unit Definition: 1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37°C in a 50 μL system.Please contact us at for specific academic pricing.
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- Properties
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Overview