FuniCut™ Bsa I GMP-grade

FuniCut™ Bsa I GMP-grade

Catalog Number:
E001505847YEA
Mfr. No.:
10661ES03/10/60
Price:
$333
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      • Overview
        • This product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1/N5-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.

          This product is produced in accordance with GMP process requirements and provided in a liquid form.

          Expression Host: Recombinant E. coli with BasI gene
          Reaction Temperature: 37°C
          Storage Buffer: 10 mM Tris-HCl, 0.2 M NaCl, 0.1 mM EDTA, 1mM DTT, 50% Glycerol
          Unit Definition: 1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37°C in a 50 μL system.

          Please contact us at for specific academic pricing.

      • Properties
        • Storage
          This product should be stored at -25 ~ -15°C for two years.
          Note
          Heat inactivation condition: incubate at80°C for 20min.
          Please operate with lab coats and disposable gloves, for your safety.

          * For Research Use Only

      • Applications
        • Application Description
          Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
          Digestion of DNA to obtain specific sticky ends;
          Linearize plasmid template before in-vitro transcription.

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