FITC/ PE - anti Human MPO/CD22 - MPO-1/1.7,17, HIB22

Reactivity: MPO antibody reacts with the intracellular enzyme, myeloperoxidase in peripheral blood granulocytes on cytospots in a characteristic granular pattern. Monocytes are negative or slightly positive while eosinophils and lymphocytes are negative. MPO antibodies stain promyelocytes, myelocytes, metamyelocytes and mature neutrophil granulocytes in bone marrow. The CD22 monoclonal antibody is directed against the CD22-antigen, which is expressed on human B lymphocytes. Normal T-cells, polymorph nuclear cells, monocytes and platelets were found to be negative. Cell lines SB, Raji and NALM-1 were found to be positive. Most CALLA-ALL were found to be positive. The reaction pattern to CLL and NHL is variable from weak to negative. PLL is clearly positive and Hairy Cell Leukaemia is very strong positive with this antibody. Specificity: The MPO monoclonal antibody reacts with virtually all cells of the myeloid lineage (from myekloblast to neutrophils and monocytes). The monoclonal antibody does not react with human lymphocytes, thrombocytes and erythrocytes. 96% of all AML's are found positive; 100% of all ALL's are found negative. The CD22 clone HIB22 monoclonal antibody was included on the 5th International Workshop. It is a heterodimer composed of two glycoprotein chains of 130 - 140 kD molecular weight glycoprotein chains The antigen is present as both a cytoplasmic and membrane antigen on B lymphocytes. The CD22 antigen is expressed in the cytoplasm of the majority of B lymphocytes including early B progenitor cells. Antigen expression appears at an early stage in B lymphocyte differentiation at approximately the same stage as the CD19 antigen. Surface expression of the CD22 antigen by B lymphocytes is variable. Circulating B lymphocytes are CD22+ and follicular mantle and marginal zone B lymphocytes show strong binding of CBL147. Germinal centre B cells exhibit only weak expression of the CD22 antigen. The CD22 antigen is poorly expressed by non-T ALL cells and by a proportion of B- CLL cells. Hairy cell leukeamia cells have been demonstrated to strongly express the CD22 antigen.

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