FastPANGEA™ Long PCR DNA Polymerase

FastPANGEA™ Long PCR DNA Polymerase is an accurate and robust enzyme that combines Horse-Power™ Taq DNA Polymerase and a DNA proofreading Polymerase with 3’ to 5’ exonuclease activity that is optimized for PCR amplification of very long DNA templates (long range PCR). As it is already well-known, Taq DNA Polymerase is inefficient at amplifying fragments larger than 3–5 kb due to its inability to repair nucleotide mismatches following misincorporation. The addition of a small quantity of proofreading enzyme allows mismatches to be repaired and extension to continue, resulting in the amplification of long amplicons with high yield. The presence of the proofreading polymerase significantly increases fidelity (6. 5x) as compared to Taq DNA Polymerase alone. This mixture of enzymes allows for long and accurate PCR amplification of targets from a variety of templates, such as 5-15 kb of genomic DNA. It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. As well, it possesses 3´→ 5´ exonuclease activity and it generates PCR products with blunt ends and generate 3´-adenine overhang in amplified DNA and thus such Taq amplified DNA could be cloned into T-vectors.

Concentration: 5 U/μL – Includes for 200U: – 200 U FastPANGEA™ Long PCR DNA Polymerase (5 U/μl) – 1. 5 mL Buffer PA (10x) – 1. 5 mL MgCl2 (25 mM) – 50 μL DMSO (100%)
Master Mix (2x) – Includes for 200 rxn: – 200 rxn FastPANGEA™-Long PCR Master Mix (2x) – 50 μL DMSO (100%) – 100 μL MgCl2 (25 mM)
*Separate tubes of DMSO and 25 mM MgCl2 solutions are provided for further optimisation.

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