Endo S/Endo S with Ig control

This product is recombinant Endo-β-N-Acetylglucosaminidase F2 (Endo S; glycosyl hydrolase family 18, EC #3.2.1.96), cloned from Streptococcus pyogenes and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

Endo S catalyzes the cleavage of N-linked glycans from IgG heavy chain.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit definition: One unit is defined as the amount of Endo-S required to deglycosylate > 90% of 2.5 μg of human IgG in 1 h at 37°C in 25 µL 1X Reaction Buffer 6 (50 mM NaOAc, 5 mM CaCl2, pH 5.5).

Product reconstitution: Dissolve the lyophilized Endo S in 100 μL molecular grade water to make a 25,000 units/mL (Cat #GE0801) solution in enzyme storage buffer (20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5). Once reconstituted, store at 4°C for up to 7 days or -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles. The optional control substrate (Cat #GC0101) is immunoglobulin (Ig) purified from human serum by Protein A affinity chromatography.

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