Beta 1,3 Galactosidase

This product is recombinant β1,3 galactosidase (glycosyl hydrolase family GH35, EC #3.2.1.23), cloned from Streptococcus pneumoniae and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEV™ (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

This product catalyzes the hydrolysis of terminal β1,3-linked galactose (Gal) from oligosaccharides and glycoprotein substrates.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit definition: One unit is defined as the amount of β1,3 Galactosidase required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-β-D-galactopyranoside (pNP-Gal) in 10 min at 37°C in 100 μL 1X Reaction Buffer 1 (20 mM Tris, 50 mM NaCl, 1 mM EDTA pH 7.5).

Product reconstitution: Dissolve the lyophilized product in 100 μL molecular grade water to make a 20,000 units/mL (Cat #GE0901) solution in enzyme storage buffer (20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5). Once reconstituted, store at 4°C for up to 7 days or -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

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