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Comparing the cellular expression levels of EGFP mRNA, LNP-encapsulated mRNA demonstrated higher expression compared to EGFP mRNA delivered by a commercial transfection reagent. 1 ug of EGFP mRNA was transfected into cells followed by (A) Flow cytometry analysis of EGFP expression in Jurkat and 293T cells and (B) Fluorescent imaging of 293T cells 24 hours post-transfection. Nucleotides of EGFP mRNA are both unmodified. Median Fluorescence Intensity (MFI).

Representative quality control (QC) results of EGFP LNP-mRNA. (A) Particle size was determined by dynamic light scattering (DLS) which measures the intensity differences of fluctuated light due to motion of particles. The polydispersity index (PDI) reflects the heterogeneity of a sample on particle size. (B) Zeta potential reflects the stability of LNP. The Zeta potential of the sample was between -1.872 mV and +1.872 mV.

EGFP LNP-mRNA (unmodified)

EGFP LNP-mRNA (unmodified)

Catalog Number:

TR02457012VEC

Mfr. No.:

NL1010-0100; NL1010-1000

Price:

$1,806

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- Overview
  - Our EGFP LNP-mRNA has been sequence optimized, fully QC'ed, and functionally validated. It can be used as a control for both in vivo and in vitro applications. Of note, the encapsulated EGFP IVT mRNA was generated with or without modified nucleotide.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    LNP mRNA
    
    Storage
    
    Our LNP-mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 6 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
    
    * For research use only.

Documents

LNP-mRNA for in vitro applications

LNP-mRNA for in vivo applications

mRNA gene delivery solutions

RNA from design to therapy

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