ECOS™ Super Blue Competent Cells [XL1-Blue]

ECOS™ Super Blue Competent Cells [XL1-Blue]

Catalog Number:
CC02547418YEA
Mfr. No.:
FYE109-10V; FYE109-80VL
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      • Overview
        • XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.

          Features
          • A strain modified to be ideal for blue/white screening
          • Tetracycline resistant

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Other Properties
          Efficiency: >2 x 10⁹ cfu/μg
          Control Plasmid (pUC19): 5μl (10⁻⁴ μg/μl)
          Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’proABlacIqZDM15 Tn10 (TetR)]
          Storage
          -70°C

          * For Research Use Only.

      • Applications
        • Application Description
          • Routine cloning application
          • Subcloning
          • cDNA library
          • Blue/white screening

    Documents

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