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Overview
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DNase I (deoxyribonuclease I, RNase-free) is derived from a recombinant E. colistrain carrying Bovine Pancreatic DNase I. It is an endonuclease that nonspecifically cleaves DNA to release ditri- and oligonucleotide products with 5´ phosphorylated and 3´-hydroxylated ends. This enzyme was originally isolated from bovine pancreas, and the mammalian pancreas is one of the dominating sources until this day. DNase I act on various DNAs such as single and double-stranded DNA, RNA: DNA hybrids, and chromatin (the cleavage rate is affected by histones).
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Overview