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DNA Polymerase I, Large (Klenow) Fragment

DNA Polymerase I, Large (Klenow) Fragment

Catalog Number:

PER1268508CAN

Mfr. No.:

P0041

Price:

$319

Size:

500 U

Quantity:

Add to Cart:

- Overview
  - Klenow Fragment is the Large Fragment of DNA Polymerase I. It shows 5’→3′ polymerase activity and 3’→5′ exonuclease (proofreading) activity, but lacks 5’→3′ exonuclease activity of DNA Polymerase I.
    
    Includes for 500 U:
    – 50 μL Klenow Fragment (10 U/μL)
    – 1 mL Reaction Buffer (10x)
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    T4 DNA Polymerase
    
    Storage
    
    -20 °C.
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application
    
    End-Point PCR
    
    Application Description
    
    DNA sequencing, fill-in of 5’ overhangs and removal of 3’ overhangs to form blunt ends and second strand synthesis in mutagenesis.
    DNA blunting by fill-in of 5’-overhangs or removal of 3‘-overhangs.
    Random-primed DNA labeling.
    Labeling by fill-in 5’-overhangs of dsDNA.
    DNA sequencing by the Sanger method.
    Site-specific mutagenesis of DNA with synthetic oligonucleotides.
    Second strand synthesis of cDNA.

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