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DNA Polymerase I (E. coli)

DNA Polymerase I (E. coli)

Catalog Number:
PER1268510CAN
Mfr. No.:
P0040
Price:
$281
  • Size:
    500 U
    Quantity:
    Add to Cart:
    Add to Cart
      • Overview

        • DNA Polymerase I is a multifunctional enzyme that combines a DNA polymerase activity, a 5´→ 3´exonuclease activity and a 3’→5′ proofreading exonuclease activity. The 5´→3´ exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labelling DNA by nick translation.

          Includes for 500 U:
          – 50 μL DNA Polymerase I (10 U/μL)
          – 1 mL Reaction Buffer (10x)

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Related Polymerases
          Storage
          -20 °C.

          * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.

      • Applications
        • Application
          End-Point PCR
          Application Description
          High-percentage incorporation of radioactivity for nick translation assays.
          Manufacturing of alternating copolymers such as poly d(A-T) and homopolymers such as poly dG-poly dC.
          Klenow fragment: DNA sequencing, fill-in of 5’ overhangs and removal of 3’ overhangs to form blunt ends and second strand synthesis in mutagenesis.
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