Direct cAMP ELISA 5 Plate

This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution.

Sensitivity increased 10-fold by acetylation (reagents included)
Absorbance read at 405 nm
Large signal-to-background ratio offers superior sensitivity

The Direct cAMP ELISA 5 Plate kit allows you to easily assess adenylyl cyclase activation by GPCRs.
Cited sample types include cell culture supernatant1, and tissue culture2.1. Effect of Tumor Necrosis Factor-{{alpha}}, Interferon-{{gamma}}, and Transforming Growth Factor-{beta} on Adipogenesis and Expression of Thyrotropin Receptor in Human Orbital Preadipocyte Fibroblasts: R. W. Valyasevi, et al.; J. Clin. Endocrinol. Metab. 86, 903 (2001)2. Cyclic Adenosine Monophosphate Regulation of Ion Transport in PorcineVocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008)

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