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Overview
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DAPI is a nuclear staining reagent that can stain DNA. It emits blue fluorescence and its brightness increases by about 20 times after it intercalates into double-stranded DNA. DAPI is commonly used for the detection of apoptosis. After staining, it can be observed with a fluorescence microscope or detected by flow cytometry. DAPI has a high tolerance to photobleaching and can be used to detect mitochondrial DNA in yeast, chloroplast DNA, viral DNA, and chromosomal DNA. At a low concentration (1 µg/mL), DAPI is impermeable to live cells but can be used as a nuclear staining agent for fixed cells or tissue sections. At a high concentration (10 µg/mL), DAPI can be used to stain live cells.
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