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Figure 1. Immunohistochemical identification of Dopamine Receptor 2 in hypothalamus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected on a distinct population of cells in the neuroendocrine tumor.

Figure 2. Immunohistochemical identification of Dopamine Receptor 1 and Dopamine Receptor 2 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-IC) at a dilution of 1:100 (left panel) or incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100 (right panel). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of Dopamine Receptor 2 in human neuroendocrine tumor. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected on a distinct population of cells in the neuroendocrine tumor.

Figure 4. Immunohistochemical identification of Dopamine Receptor 2 in human small cell lung carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected on a distinct population of cells in the small cell lung carcinoma.

Figure 5. Immunohistochemical identification of Dopamine Receptor 2 in human pancreatic islets. Pancreatic sections were dewaxed, microwaved in citric acid, and incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, D2 receptors were detected in pancreatic islets.

Figure 6. Immunocytochemical identification of Dopamine Receptor 2 in transfected HEK293 cells. HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were either not exposed or exposed to 100 nM Quinpirole for 30 min and immunocytochemically stained with anti-D2 antibody (7TM0215N-IC) at a dilution of 1:200. Note, D2 receptors were confined to the plasma membrane in untreated cells (0 min). D2 receptors were seen in perinuclear clusters of vesicles after 30 min Quinpirole exposure.

Figure 7. Validation of the Dopamine Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Dopamine Receptor 2 (D2) were lysed and immunoblotted with the phosphorylation-independent anti-D2 antibody (7TM0215N-WB) at a dilution of 1:1000.

D2 ( IHC-grade ), Dopamine Receptor 2 Polyclonal Antibody

D2 ( IHC-grade ), Dopamine Receptor 2 Polyclonal Antibody

Catalog Number:

A001455985TMA

Mfr. No.:

7TM0215N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The D2 receptor antibody is directed against the third intracellular loop of mouse, rat and human D2 dopamine receptor. It detects both the long and short form of D2. It can be used to detect total D2 receptors in Western blots independent of phosphorylation. The D2 antibody can also be used to isolate and enrich D2 receptors from brain lysates. It also detects D2 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic peptide presents carboxyl-terminal tail of human D2
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  D2, D2R, Dopamine Receptor 2 (UniProt:P14416 (human), P61168 (mouse), P61169 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB; ICC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200

Reference
- Mann A, Keen AC, Mark H, Dasgupta P, Javitch JA, Canals M, Schulz S, Robert Lane J. New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling. Sci Rep. 2021 Apr 15;11(1):8288. doi: 10.1038/s41598-021-87417-2. PMID: 33859231; PMCID: PMC8050214.

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