Cytidine (n-bz) 3'-tBDSilyl CED phosphoramidite

3'-TBDMS-2'-CE Amidite, 2'-5' Linked RNA

C52H66N5O9PSi

964.170

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

Synthesis of Unnatural RNA 2’-5’-linked Oligos:

We offer a wide variety of 3’-O-TBDMS protected ribonucleoside phosphoramidites that allow the synthesis of 2’-5’-linked oligos. The low molecular weight oligomer 5’-O-triphosphoryl adenylyl-(2’-5’)-adenylyl-(2’-5’)-adenosine (pppA2’p5’A2’p5’A) activates the latent endo-ribonuclease RNase L, which subsequently cleaves the messenger and ribosomal RNAs.1
Such oligonucleotides are very useful to determine their exact biological role, to extend their biological half life, or to alter the biological activity of the core structure (A2’p5’A2’p5’A).2
2’,5’-linked synthetic RNA sequences consisting of mixed bases and their duplexes are not substrates of the enzyme RNase H (Escherichia coli or HIV-1 reverse tran­scriptase). However, they can inhibit the RNaseH mediated cleavage of a natural DNA/RNA substrate.3

References:

1. Charubala, R.; Uhlmann, E.; Himmelsbach, F.; Pfleiderer, W. Helv. Chem. Acta 1987, 70, 2028.

2. Jamoulle, J. C.; Imai, J.; Lesiak, K.; Torrence, P. F. Biochemistry, 1984, 23, 3063.

3. Wasner, M.; Arion, D.; Borkow, G.; Noronha, A.; Uddin, H. J.; Parniak, M. A.; Damha, M. J. Biochemistry 1998, 37, 7478-7486.