Name: CyFlow™ Lck Alexa Fluor™ 647
Price: 536.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its ζ subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    LCK-01
    
    Immunogen
    
    Peptide corresponding to amino acids 22-36 in the sequence of human Lck
    
    Conjugate
    
    Alexa-fluor®-647
    
    Formulation
    
    The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Concentration
    
    1 mg/mL
    
    Regulatory Status
    
    RUO
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
    
    Emission Maximum: 665 nm
    Excitation Maximum: 650 nm