Name: CyFlow™ IgM Purified
Price: 306.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgM
    
    Clone
    
    PFR-03
    
    Conjugate
    
    Purified
    
    Formulation
    
    The reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Concentration
    
    1 mg/mL
    
    Regulatory Status
    
    RUO
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry (isotype control)
    
    Application Description
    
    The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10·µg/ml of incubation mixture), change the experimental conditions to reduce the background.