Name: CyFlow™ Helios PE
Price: 488.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Helios (IKZF2; Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.
- Properties
  - Host
    
    hamster
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG
    
    Reactivity
    
    human; mouse
    
    Clone
    
    22F6
    
    Immunogen
    
    Peptide coresponding to the amino acids 51-107 of Helios
    
    Conjugate
    
    PE
    
    Formulation
    
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    IKZF2
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    
    Emission Maximum: 576 nm
    Excitation Maximum: 496 nm, 565 nm