Name: CyFlow™ CD3 Biotin
Price: 378.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG2a
    
    Reactivity
    
    human
    
    Clone
    
    MEM-57
    
    Immunogen
    
    Human thymocytes and T lymphocytes
    
    Conjugate
    
    Biotin
    
    Formulation
    
    The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Concentration
    
    1 mg/mL
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Leu4, T3
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 3.3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.