CyFlow™ CD229 PE

CyFlow™ CD229 PE

Catalog Number:
A001343393SYS
Mfr. No.:
AN938951
Price:
$488
  • Size:
    100 tests (2.0 mL)
    Quantity:
    Add to Cart:
      • Overview
        • Please contact us at for specific academic pricing.

          Background

          CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

      • Properties
        • Host
          mouse
          Antibody Type
          monoclonal
          Isotype
          IgG1
          Reactivity
          human
          Clone
          HLy9.25
          Immunogen
          CD299-transfected 300.19 pre-B cell line
          Conjugate
          PE
          Formulation
          The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
          Storage
          Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
          Regulatory Status
          RUO
          Antigen
          hly9, Ly-9 , mLY9, SLAMF3

          * Research use only

      • Applications
        • Application
          Flow Cytometry
          Application Description
          The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

          Emission Maximum: 576 nm
          Excitation Maximum: 496 nm, 565 nm

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