Cyclic AMP (cAMP) Activity Fluorometric Assay (TBS2081)

This assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with HRP-cAMP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive HRP fluorescence substrate is added to generate fluorescence can be measured at excitation 535 nm and emission at 585 nm. Because of the competitive nature of this assay, the fluorescent intensity is inversely proportional to the quantity of sample cAMP. Measurement using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay is more sensitive than a colorimetric assay.

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