Name: Cyanine 3-dCTP
Price: 287.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Cy3-dCTP can be used for direct enzymatic labeling of DNA/cDNA by PCR with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, cDNA labeling and 3’-end labeling. Cy3-dCTP can replace dCTP in reactions in which it serves as a substrate for T4 and Taq DNA polymerases, E. coli DNA polymerase (holoenzyme and Klenow fragment), reverse transcriptase (from AMV and M-MuLV) and terminal transferase. The synthesized dye-labeled DNA/cDNA probes can be used for the identification of specific sequences by in-situ hybridization, microarray or blotting techniques. Cy3-dCTP can also be used for multicolor fluorescence labeling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Cy3-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Cy3-dCTP / 50% dCTP.
- Properties
  - Categories
    
    Direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation
    
    Alternative Name
    
    Cyanine 3-5-Propargylamino-2'-deoxycytidine-5'-Triphosphate,Cy3-dCTP; tetralithium mono(2-((1E,3E)-3-(1-(6-((3-(4-amino-1-(4-hydroxy-5-(((oxido((oxido(phosphonatooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2-oxo-1,2-dihydropyrimidin-5-yl)prop-2-yn-1-yl)amino)-6-oxohexyl)-3,3-dimethyl-6-sulfonatoindolin-2-
    
    Molecular Formula
    
    C43H54N6O20P3S2 (free acid)
    
    Molecular Weight
    
    1,131.9 (free acid)
    
    Appearance
    
    Solution
    
    Purity
    
    ≥95% by AX-HPLC
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    NC(C(C#CCNC(CCCCCN1C2=C(C=CC(S(=O)([O-])=O)=C2)C(C)(C)/C1=C\C=C\C3=[N+](CC)C(C=C(S(=O)([O-])=O)C=C4)=C4C3(C)C)=O)=CN5C6CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O6)=NC5=O.[Li+].[Li+].[Li+].[Li+]
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Cyanine 3-dCTP

Cyanine 3-dCTP

Background

Documents

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