Cortical Glutamatergic Neurons - Human iPSC-Derived

Origin: Human iPSC line

Contents: 1 vial of cells and corresponding supplements

GFP-expressing neurons also available
Cryopreserved

Marker Expression
Cortical Glutamatergic Neurons (BX-0300) have high neuronal purity (>90%) and comprise predominantly excitatory neurons. Labeling with the pan-neuronal marker MAP2 (red) highlights the neuronal purity of these cells. Labelling with the glutamatergic-specific markers CTIP2 (green) and TBR1 (blue) indicates overwhelming presence of glutamatergic neurons.

Morphology
Cortical Glutamatergic Neurons (BX-0300) exhibit substantial neurite outgrowth within a week in culture and are adherent. Calcein staining (green) demonstrates the characteristic oval cell shape and long processes of Cortical Glutamatergic Neurons in culture.

Function
Cortical Glutamatergic Neurons (BX-0300) exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Calcium Influx Assays: Changes in calcium concentration are closely tied to neuronal activity as action potentials are associated with large pre-synaptic calcium influx and a notable rise in postsynaptic calcium at excitatory synapses. This can be observed experimentally by stimulating the neurons or culturing the neurons under suitable conditions to form mature networks that exhibit spontaneous oscillations. The influx of calcium can be measured using a variety of calcium-sensitive fluorescent dyes, which are commercially available.

MEA Assays: Multi-electrode arrays (MEA) measure extracellular voltage changes that occur as neurons fire action potentials. These measurements reveal the firing patterns of individual neurons as well as the patterns of neuronal networks that exist in the cell culture. Such measurements are non-invasive and allow for repeated recordings.

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