CM SEPLITE® FF Chromatographic Media

Agarose matrix, CM group, fast flow, Equivalent to CM Sepharose FF

• Column selection: In theory, as long as the column is long enough, you can get the ideal resolution, but because the column flow rate is related to the pressure gradient, increasing the column length makes the flow rate slower, the peak broadens, and resolution Decrease; the diameter of the column increases, so that the unevenness of the liquid flow increases, and the resolution decreases significantly. A
• The pH and ionic strength of the elution buffer must be strictly controlled during the purification process. The sample and CM chromatography medium must be thoroughly equilibrated with elution buffer before column chromatography. A
• The installed column bed must be flat and free of channels and bubbles, otherwise it should be reinstalled.
• The flow rate should be strictly controlled during the elution, and not too fast.
• The volume of the sample should be small, and the concentration should not be too high.
• Prevent the cylinder from drying out during sample addition and the entire elution process.

Agarose Chromatography Resin