Name: cGAS Polyclonal Antibody (1mg/mL) for WB
Price: 416.00 USD

Overview
- Calculated MW: 113kDa
  Observed MW: 114kDa
  
  Please contact us at for specific academic pricing.
  
  Background
  
  Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity (PubMed:23258413, PubMed:23707061, PubMed:23722159, PubMed:24077100, PubMed:25131990, PubMed:29976794, PubMed:30799039). Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p] (PubMed:28363908, PubMed:28214358). Acts as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses (PubMed:28363908). Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production (PubMed:28363908, PubMed:28314590). Preferentially recognizes and binds curved long DNAs (PubMed:30007416). In contrast to other mammals, human CGAS displays species-specific mechanisms of DNA recognition and produces less cyclic GMP-AMP (cGAMP), allowing a more fine-tuned response to pathogens (PubMed:30007416). Has antiviral activity by sensing the presence of dsDNA from DNA viruses in the cytoplasm (PubMed:28363908). Also acts as an innate immune sensor of infection by retroviruses, such as HIV-1, by detecting the presence of reverse-transcribed DNA in the cytosol (PubMed:23929945). Detection of retroviral reverse-transcribed DNA in the cytosol may be indirect and be mediated via interaction with PQBP1, which directly binds reverse-transcribed retroviral DNA (PubMed:26046437). Also detects the presence of DNA from bacteria, such as M.tuberculosis (PubMed:26048138). cGAMP can be transferred from producing cells to neighboring cells through gap junctions, leading to promote TMEM173/STING activation and convey immune response to connecting cells (PubMed:24077100).
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide of human cGAS
  
  Purification
  
  Affinity purification
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% sodium azide,50% glycerol,pH7.3.
  
  Storage
  
  Store at -20°C Valid for 12 months. Avoid freeze-thaw cycles.
  
  Concentration
  
  1mg/mL
  
  Antigen
  
  MB21D1, C6orf150, cGAS, h-cGAS, cyclic GMP-AMP synthase (Gene ID:115004; UniProt:Q8N884)
  
  * For reference only.

Applications

Application

WB

Application	Dilutions
WB	1:500-1:2000

cGAS Polyclonal Antibody (1mg/mL) for WB

cGAS Polyclonal Antibody (1mg/mL) for WB

Background

We Also Recommend

ANKRD52 Polyclonal Antibody

eIF4EBP1 Polyclonal Antibody (1mg/mL) for IF

SIRT1 Polyclonal Antibody (1mg/mL) for WB