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Overview
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For analyzing mammalian cell samples as HTS
Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.
In samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.
▶ Intrusments
The stable light makes it possible to manually add the ATP Reagent and the ATP Standard with a multi-channel pipette or to use a microplate luminometer with dispensers.
▶ Assay Procedure
Detailed described procedures are found in the "Instructions for Use", which is included with every kit.Please contact us at for specific academic pricing.
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- Properties
- Applications
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Overview