-Cellstain-DAPI

- Overview
  - DAPI is an AT-sequence-specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. DAPI is not permeable through viable cell membranes, but it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photobleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA, and chromosomal DNA. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
    
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- Properties
  - Categories
    
    Cell Staining
    
    CAS Number
    
    28718-90-3
    
    Molecular Formula
    
    C16H17Cl2N5
    
    Molecular Weight
    
    350.25
    
    Appearance
    
    Yellow powder or solid
    
    Storage
    
    -20°C, protect from light
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application Description
    
    Staining Procedure
    1.Prepare 10-50 μM DAPI solution with PBS or an appropriate buffer.a)
    2.Add DAPI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
    3.Incubate the cell at 37°C for 10-20 min.
    4.Wash cells twice with PBS or an appropriate buffer.
    5.Observe the cells using a fluorescence microscope with 360 nm excitation and 460 nm emission filters.
    a) Since DAPI may be carcinogenic, extreme care is necessary during handling.
    b) Or you may replace the culture medium with 1/10 concentration of DAPI buffer solution.
- Reference
  - 1) W. Schnedl, et al., DIPI and DAPI: Fluorescence Banding with Only Negligible Fading. Hum Genet. 1977;36:167-172.
    
    2) I. W. Taylor, et al., An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations. J Histochem Cytochem. 1980;28:1224-1232.
    
    3) F. Otto, et al., A Comparative Study of DAPI, DIPI, and Hoechst 33258 and 33342 as Chromosomal DNA Stains. Stain Technol. 1985;60:7-11.
    
    4) N. Poulin, et al., Quantitative Precision of an Automated Image Cytometric System for the Measurement of DNA Content and Distribution in Cells Labeled with Fluorescent Nucleic Acid Stains. Cytometry. 1994;16:227-235.
    
    5) M. Kawai, et al., Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process. J Appl Microbiol. 1999;86:496-504.

MSDS

-Cellstain-DAPI

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