CellQuanti-Blue™ Cell Viability Assay Kit

- Overview
  - This homogeneous assay involves simply adding a single reagent, the CellQuanti-Blue™ reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 – 570 nm, emission wavelength = 590 – 620 nm) after an incubation step. The CellQuanti-Blue™ reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent,but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The CellQuanti-Blue™ reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.
    
    Key Features
    ▪ Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
    ▪ Sensitive and accurate. As low as 100 cells can be accurately quantified.
    ▪ Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day.
    ▪ Homogeneous and convenient. A single reagent and “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: FL530/590nm
    Samples: Cell culture
    Species: All
    Procedure: Assay takes 1-5 hrs, hands-on time 30 min
    Detection Limit: 100 cells
    Shelf Life: 12 months
    
    Storage
    
    4°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.
- Reference
  - Ibrihim, MIM et al (2014). Aqueous extract of Corchorus olitorius decreases cytotoxicity of aflatoxin B atoxin B1and fumonisin B and fumonisin B1 in H4IIE- in H4IIE-luccells.Hepatoma Research. 1(2) Assay: Cell viability in rat hepatoma cells.
    Fujimoto, M et al (2011). Examination of Cell Viability using the primary culture system of skin fibroblasts. XXXXX. Assay: Cell viability in human fibroblasts.
    Hemendinger, RA et al (2011). Methyl Vitamin B12 but not methylfolate rescues a motor neuron-like cell line from homocysteine-mediated cell death. Toxicol Appl Pharmacol. 251(3):217-25. Assay: Cell viability in human cell lines.
    Hahn, A et al (2010). Therapeutic window for bioactive nanocomposites fabricated by laser ablation in polymer-doped organic liquids. Adv. Eng. Mater. 12(5). Assay: Cell viability in mouse cell lines.
    Kelley, KD et al (2010). YPEL3, a p53-regulated gene that induces cellular senescence. Cancer Res. 70(9):3566-75. Assay: Cell viability in human U2OS-TetR cells.
    Martino, CF et al (2010). Effects of weak static magnetic fields on endothelial cells. Bioelectromagnetics 31(4):296-301. Assay: Cell viability in human endothelial cells.
    Mitsui, R et al (2010). Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells. Arch Oral Biol. 55(12):963-9. Assay: Cell viability in rat cells.
    Ramirez, CN et al (2010). Cell viability assessment: toward content-rich platforms. Expert Opinion on Drug Discovery 5(3): 223-233. Assay: Cell viability in human cell line.
    Wang, T et al (2010). Activated T-cells inhibit neurogenesis by releasing granzyme B: rescue by Kv1.3 blockers. J Neurosci. 30(14):5020-7. Assay: Cell viability in human neural progenitor cells.
    Heminger, K et al (2009). Alterations in gene expression and sensitivity to genotoxic stress following HdmX or Hdm2 knockdown in human tumor cells harboring wild-type p53. Aging 1(1):89-108. Assay: Cell viability in human cell lines.
    Nakajima, Y et al (2009). Neuroprotective effects of Brazilian green propolis and its main constituents against oxygen-glucose deprivation stress, with a gene-expression analysis. Phytother Res. 23(10):1431-8. Assay: Cell viability in rat cell line.
    Zayas-Santiago A, Kang Derwent JJ (2009). Preservation of intact adult rat photoreceptors in vitro: study of dissociation techniques and the effect of light. Mol Vis.15:1-9. Assay: Cell viability in rat photoreceptor cells.
    Desplats, PA et al (2008). Functional roles for the striatal-enriched transcription factor, Bcl11b, in the control of striatal gene expression and transcriptional dysregulation in Huntington's disease. Neurobiol Dis. 31(3):298-308. Assay: Cell viability in human cells.
    Hemendinger, RA et al (2008). Huperzine A provides neuroprotection against several cell death inducers using in vitro model systems of motor neuron cell death. Neurotox Res. 13(1):49-61. Assay: Cell viability in rat cells.
    Vijaykumar, TS et al (2008). Chloroquine mediated molecular tuning of astrocytes for enhanced permissiveness to HIV infection. Virol. 381(1):1-5. Assay: Cell viability in human cells.
    Mochida, S et al (2007). Geranylgeranylacetone Ameliorates Inflammatory Response to Lipopolysaccharide (LPS) in Murine Macrophages: Inhibition of LPS Binding to The Cell Surface. J. Clin. Biochem. Nutr., 41, 115-123. Assay: Cell viability in mouse cell lines.

MSDS

Documents

Protocol

CellQuanti-Blue™ Cell Viability Assay Kit

CellQuanti-Blue™ Cell Viability Assay Kit

Documents

We Also Recommend

CellQuanti-MTT™ Cell Viability Assay Kit

EnzyChrom™ Acetaldehyde Assay Kit

EnzyChrom™ Acetate Assay Kit