Calcein Blue

- Overview
  - Calcein Blue is a fluorescent metal indicator in EDTA titration. It is highly soluble in alkaline solutions, but slightly soluble in water. Its proton dissociation constants are reported to be pKa1=2.45, pKa2=7.24 and pKa3=11.3. Calcein Blue solution emits bright blue fluorescence (λem=445 nm) at pH 4-11, but no fluorescence at pH 12 or higher. The blue fluorescence of the Calcein Blue solution at pH 4-11 is quenched by metal ions such as Co, Cu, Mn, Ni, and Pb. However, Calcein Blue forms a highly fluorescent complex with alkaline earth metals at pH 12 or higher. Since the endpoint of the chelate titration is clear by UV irradiation at 200-370 nm in a dark room, highly stained samples can be used. Calcein Blue is also suitable to determine Fe and Zr ions. Calcein Blue-Zn chelate can detect 10 ppb of F-. Calcein Blue aqueous solution emits laser at 449-490 nm.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Ion Analysis
    
    Alternative Name
    
    8-[N,N-Bis(carboxymethyl)aminomethyl]-4-methylunbelliferone
    
    CAS Number
    
    54375-47-2
    
    Molecular Formula
    
    C15H15NO7
    
    Molecular Weight
    
    321.28
    
    Appearance
    
    White or pale yellow powder
    
    Storage
    
    ambient temperature, protect from light
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) D. H. Willkins, Calcein Blue - A New Metalfluorechromic Indicator for Chelatometric Titrations. Talanta. 1960;4:182-184.
    
    20 J. H. Eggers, Umbellikomplexon Und Xanthokomplexonein Beitrag zur Kenntnnis Komplexometrischer Fluoreszensindikatoren. Talanta. 1960;4:38-43.
    
    3) H. N. Elsheimer, Complexometric Determination of Gallium with Calcein Blue as Indicator. Talanta. 1967;14:97-102.
    
    4) M. A. Salam Khan, et al., A Structural Study of the Methyleneiminodiacetic Acid Derivatives of Some 7-Hydroxycoumarins. Anal Chim Acta. 1968;43:153-156.
    
    5) R. V. Hems, et al., Spectrofluorimetric Determination of Submicrogram Amounts of Zirconium with Calcein Blue. Anal Chem. 1970;42:784-787.
    
    6) T. L. Har, et al., Spectrofluorimetric Determination of Traces of Fluoride Ion by Ternary Complex Formation with Zirconium and Calcein Blue. Anal Chem. 1971;43:136-139.
    
    70 T. Imasaka, et al., Characteristics of Coordination Compounds of Calcein Blue for a Tunable Oraganic Liquid Laser. Bull Chem Soc Jpn. 1976;49:2687-2695.
    
    8) K. A. Matsoukas, et al., Spectrofluorimetric Determination of Magnesium in Blood Serum with Calcein Blue. Anal Chim Acta. 1989;227:211-218.

MSDS

Calcein Blue

We Also Recommend

BT

Calcein

Cyanoline Blue