Name: Calbindin D9K - ¹⁵N
Price: 774.00 USD

- Overview
  - MW = 8.5 kDa calculated. Calbindin D9K (Protein S100-G, CABP, Vitamin D-dependent calcium-binding protein, Intestinal) cloned from bovine cDNA, expressed in E. coli. The protein consists of Calbindin D9K, mutant P47M(residues 5-79, UniProtKB accession P02633) with 2 Ca2+ ions bound.
    
    · Characteristics
    Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2 mM.
    
    · Assignments Available
    1H, 13C, 15N, item 201CAB-0: J.Am.Chem.Soc. 123: 4181-4188, 2001; J.Biomol.NMR 21: 85-98, 2001
    1H, 13C, 15N, item 201CAB-Ce: J.Am.Chem.Soc. 123: 4181-4188, 2001; ChemBioChem 2: 550-558, 2001
    1H, 15N, items 201CAB-La, 201CAB-Nd, 201CAB-Pr, 201CAB-Sm, 201CAB-Eu, 201CAB-Tb, 201CABDy, 201CAB-Ho, 201CAB-Er, 201CAB-Tm, 201CAB-Yb: J.Am.Chem.Soc. 123: 4181-4188, 2001; Eur.J.Inorg.Chem. 2121-2127, 2002
    
    Labeling: 15N
    NMR Standard: Bulk sample
    Shipping in Dry Ice: no
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Bovine
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
    
    Formulation
    
    1.0 mg/mL solution in ammonium acetate 50 mM pH 6.0. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 1280 M^-1cm^-1 calculated).
    (Unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
    
    Storage
    
    -20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
    NMR USE: after the experiments return the sample at 4°C.
    
    Molecular Weight
    
    8.5 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    10 20 30 40 50 60 70
    M-KSPEEL KGIFEKYAAK EGDPNQLSKE ELKLLLQTEF PSLLKGPSTL DELFEELDKN GDGEVSFEEF QVLVKKISQ
    
    * This product is intended for research use or further manufacturing only.

Calbindin D9K - ¹⁵N

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