C80EZ®-NEURON Mammalian Neuron Cryopreservation Media

The development of C80EZ® media is based on an invention that applies unique biocompatible polymer combinations to promote nanoscale cubic ice formation. The unique approach minimizes the size of hexagonal ice crystals (the dominating ice structure during freezing of normal aqueous solutions) during freezing and significantly improves the thermal stability of the frozen samples by preventing hexagonal ice regrowth or secondary growth (i.e., recrystallization) during storage and warming. Consequently, all C80EZ® products significantly reduce mechanical damage of tissue structures and fragile cell types, which are majorly associated with ice formation and regrowth during cryopreservation procedures. The antiapoptosis agents and antioxidants in the C80EZ® media also reduce cell loss due to the use of cell permeating cryoprotectants (e.g., DMSO and glycerol) and excessive intracellular water loss during freezing. Use of C80EZ® hence significantly improves the post-thaw viability and functionality of multiple cell and tissue types that are traditionally difficult to cryopreserve.
With the unique ice structural modification mechanism, application of C80EZ® products also realize long-term storage of cells and tissues in regular laboratory deep freezers (approx. -70 to -80°C ), and do not require liquid nitrogen facilities (approximately -120°C in the vapor phase and -196°C in the liquid phase for storage). If the C80EZ® products are used to store cells and tissues in liquid nitrogen facilities, the samples can be safely transported by dry ice boxes (at -78°C on dry ice surface) instead of using highly expensive and heavy liquid nitrogen dry shippers or dewars.
C80EZ®-NEURON is specially designed for mammalian primary neurons, for which traditional approaches work poorly. It can be used to preserve neurons for both -80°C and liquid nitrogen storage.
All C80EZ® products are serum free and animal protein free. For storage of C80EZ® itself, C80EZ® should be stored at 2 - 8 °C.

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