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Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 20 in mouse spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR20 (G Protein-coupled Receptor 20) antibody (7TM0091N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR20 receptors were detected at the plasma membrane of cells.

Figure 2. Western blot analysis of G Protein-coupled Receptor 20 in mouse tissues in vivo. Mouse tissues were dissected, homogenated and immunoblotted using anti-GPR20 (G protein-coupled receptor 20) antibody (7TM0091N-IC) at a dilution of 1:1000. Note, absence of GPR20 receptors in kidney, small intestine and liver but strong presence colon and stomach and slightly decteable in heart.

Figure 3. Immunocytochemical identification of G Protein-coupled Receptor 20 in HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 20 (GPR20) were immunocytochemically stained with anti-GPR20 (G protein-coupled receptor 20) antibody (7TM0091N-IC) at a dilution of 1:200. Note, GPR20 receptors were confined to the plasma membrane in transfected cells.

Figure 4. Western blot analysis of the G Protein-coupled Receptor 20 in mouse tissues in vivo. Tissues from GPR20-deficient mice (GPR20-KO) and their wild-type littermates (GPR20-WT)were dissected, homogenated and immunoblotted using anti-GPR20 (G protein-coupled receptor 20) antibody (7TM0091N-IC) at a dilution of 1:1000. Note, absence of GPR20 receptors in colon in GPR20-KO but not in GPR20-WT mice.

mGPR20 ( IHC-grade ), G Protein-Coupled Receptor 20 Polyclonal Antibody ( Mouse )

mGPR20 ( IHC-grade ), G Protein-Coupled Receptor 20 Polyclonal Antibody ( Mouse )

Catalog Number:

A001455993TMA

Mfr. No.:

7TM0091N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The GPR20 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse GPR20. It can be used to detect total GPR20 receptors in Western blots independent of phosphorylation. The GPR20 antibody can also be used to isolate and enrich GPR20 receptors from tissue lysates. It also detects GPR20 in cultured cells and tissue sections by immunohistochemistry. The GPR20 antibody has been validated using knockout mice (KO-Validated).
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  mouse
  
  Immunogen
  
  A synthetic peptide presents carboxyl-terminal tail of mouse GPR20
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  GPR20, G Protein-coupled Receptor 20 (UniProt:Q8BYC4)
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

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