Name: Bulk anti-Human CD45 antibody (BC8) - Low Endotoxin
Price: 680.00 USD

- Overview
  - Antigen Distribution: CD45 is expressed on all hematopoietic cells other than mature erythrocytes and platelets.
    Specificity: Human CD45
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
    Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    BC8
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
    
    Storage
    
    This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    1mg/ml
    
    Regulatory Status
    
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, LCA, T200, Ly5)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Functional Assays

Bulk anti-Human CD45 antibody (BC8) - Low Endotoxin

Bulk anti-Human CD45 antibody (BC8) - Low Endotoxin

Background

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