Name: Bulk anti-Human CD18 Antibody (1B4) - Low Endotoxin
Price: 680.00 USD

- Overview
  - Antigen Distribution: Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
    Specificity: Anti-CD18 antibody (clone 1B4) activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes.
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)
    Endotoxin: <1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes.
    
    For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively.
    
    The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems and to measure the expression of CD18 in B-ALL cell lines.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG2a kappa
    
    Reactivity
    
    human
    
    Clone
    
    1B4
    
    Immunogen
    
    Human peripheral blood lymphocytes
    
    Purification
    
    >95% by SDS-PAGE
    
    Formulation
    
    Sterile, preservative-free, solution in PBS. BSA and Azide free.
    
    Storage
    
    This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 2.0 mg/ml
    
    Regulatory Status
    
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:Integrin beta-2, integrin beta chain, beta 2, ITGB2)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Functional Assays; Flow Cytometry; IF Microscopy

Bulk anti-Human CD18 Antibody (1B4) - Low Endotoxin

Bulk anti-Human CD18 Antibody (1B4) - Low Endotoxin

Background

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