Name: Bulk anti-Human CD68 Antibody (KP1) - Low Endotoxin
Price: 680.00 USD

- Overview
  - Antigen Distribution: CD68 is found in the cytoplasm of monocytes/macrophages, fibroblasts, human peripheral blood lymphocytes, neutrophil primary and mast cell granules, large granular lymphocytes, basophils, basal epithelial cell layers, renal glomeruli, myeloid cells, endothelial cells, retinal epithelial cells, osteoblasts, fibroblast-like cells from bone marrow, and a wide-range of lymphoid neoplasms. CD68 is predominantly located in lysosomal membranes, with a small amount on the cell surface. Additionally, CD68 can be expressed in most hematopoietic cell lines by phorbol-induced differentiation; soluble CD68 can also be found in serum and urine.
    Specificity: Anti-CD68 antibody (clone KP1) activity is directed against human CD68.
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
    Endotoxin: <1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    KP1 was originally used as a pan-monocytic/macrophage marker against CD68; however, CD68 is not a macrophage specific antigen and KP1 is known to stain neutrophils and other non-macrophage-like cells. KP1 reacts against CD68 in a wide range of healthy and disease-associated (rheumatoid arthritis and osteoarthritis)5 tissues as well as a variety of neoplasms, tumor cell lines, and tumor-associated macrophages. KP1 detects a fixation-resistant epitope that is likely glycan-based which is shared by many cell types.
    
    KP1 was developed by immunizing Balb/c mice against a lysosomal fraction of human lung . Hybridoma supernatants derived from spleen cells were screened on cryostat human lung and tonsil sections as well as paraffin wax sections of lung tissue fixed in formol saline.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1 kappa
    
    Reactivity
    
    human
    
    Clone
    
    KP1
    
    Immunogen
    
    Lysosomal fraction of human lung
    
    Purification
    
    >95% by SDS-PAGE
    
    Formulation
    
    Sterile, preservative-free, solution in PBS. BSA and Azide free.
    
    Storage
    
    This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 2.0 mg/ml
    
    Regulatory Status
    
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:GP110, LAMP4, SCARD1)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Blotting; Dot Blot; Electron Microscopy; Flow Cytrometry; Immunocytochemistry; ICFC; Immunohistochemistry; IHC (paraffin); Immunoprecipitation; WB
- Reference
  - PD-1 Blockade Restores the Proliferation of Peripheral Blood Lymphocyte and Inhibits Lymphocyte Apoptosis in a BALB/c Mouse Model of CP BVDV Acute Infection

Bulk anti-Human CD68 Antibody (KP1) - Low Endotoxin

Bulk anti-Human CD68 Antibody (KP1) - Low Endotoxin

Background

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