Bsu DNA Polymerase, Large Fragment

Bsu DNA Polymerase, Large Fragment is expressed by a recombinant E. coli strain cloned from the Bacillus Subtilis DNA polymerase I gene and obtained by multi-step purification. This large fragment retains the 5 ´ → 3 ´ polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5´→3´ exonuclease domain (starting from codon 297 thus lacking the 5 ´ → 3 ´ exonuclease domain). This large fragment naturally lacks 3 ´ → 5 ´ exonuclease activity.

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