BsmBI Restriction Endonuclease (10 U/μL)(BsmBI Restriction Endonuclease Cutting Site CGTCTC(N)1/)

BsmBI Restriction Endonuclease (10 U/μL)(BsmBI Restriction Endonuclease Cutting Site CGTCTC(N)1/)

Catalog Number:
E001505843YEA
Mfr. No.:
15203ES80
Price:
$604
  • Size:
    1000 U
    Quantity:
    Add to Cart:
      • Overview
        • BsmBI is a Type IIs endonuclease that can recognize non-palindromic sequences and cut outside the recognition sequence. It is often used in Golden Gate assembly. This product provides an optimized reaction buffer to maximize the function of BsmBI. At the same time, the reaction buffer contains recombinant albumin, which can enhance the stability of multiple enzymes.

          Recognition position: 5'-CGTCTC(N)1↓-3' 3'-GCAGAG(N)5↑-5'
          Recommended reaction conditions: 1×BsmBI Buffer; incubate at 55°C.
          Inactivation conditions: Incubate at 80°C for 20 min.

          Components: BsmBI(10 U/μL) (100 μL); 10×BsmBI Buffer (1 mL)

          Please contact us at for specific academic pricing.

      • Properties
        • Storage
          Shipped with an ice pack. Store at -20°C, valid for 2 years.
          Note
          After 3 hours of incubation, no star activity was observed, but star activity may appear with delayed enzyme cleavage.
          Affected by CpG methylation, the sequences are completely overlapped and splicing is hindered.
          Affected by EcoBI methylation, sequences may overlap and splicing may be affected.
          Please operate with lab coats and disposable gloves, for your safety.

          * For Research Use Only

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