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Overview
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BsmBI is a Type IIs endonuclease that can recognize non-palindromic sequences and cut outside the recognition sequence. It is often used in Golden Gate assembly. This product provides an optimized reaction buffer to maximize the function of BsmBI. At the same time, the reaction buffer contains recombinant albumin, which can enhance the stability of multiple enzymes.
Recognition position: 5'-CGTCTC(N)1↓-3' 3'-GCAGAG(N)5↑-5'
Recommended reaction conditions: 1×BsmBI Buffer; incubate at 55°C.
Inactivation conditions: Incubate at 80°C for 20 min.
Components: BsmBI(10 U/μL) (100 μL); 10×BsmBI Buffer (1 mL)Please contact us at for specific academic pricing.
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- Properties
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Overview