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Bovine Alkaline Phosphatase Native Protein

Bovine Alkaline Phosphatase Native Protein

Catalog Number:
P001415129ABE
Mfr. No.:
Abe32-2174
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  • Size:
    5 mg
    Quantity:
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      • Overview

        • The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

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      • Properties
        • Protein Name
          EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase.
          Source
          Calf intestine
          Type
          Recombinant Proteins
          Formulation
          50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
          Storage
          AP should be stored at 4°C. Please Do-Not freeze.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          >1000 U/mg (pH 9.6, 25°C and 0.025M glycin, 10% glycerol as buffer.

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