Biotin-11-dUTP

- Overview
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    Background
    
    Biotin-11-dUTP, a biotinylated deoxynucleotide triphosphate analogue, can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart deoxythymidine triphosphate (dTTP). The resulting biotin-labeled DNA/cDNA probes are then detected with streptavidin-horseradish peroxidase (HRP) conjugates, alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Biotin-11-dUTP is commonly used for PCR, nick translation, as well as reverse transcription. It is also suitable for apoptosis assays.
    
    1. Choi JH, Kim SY, Kim SK, et al. An Integrated Approach for Analysis of the DNA Damage Response in Mammalian Cells. Journal of Biological Chemistry, 2015, 290(48): 28812-28821.
    2. Day PJ, Bevan IS, Gurney SJ, et al. Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochemical Journal, 1990, 267(1): 119-123.
- Properties
  - Categories
    
    A biotin-labeled dUTP
    
    Alternative Name
    
    lithium (5-(4-amino-2-oxo-5-((E)-3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate
    
    CAS Number
    
    1221498-88-9
    
    Molecular Formula
    
    C28H44Li4N7O16P3S
    
    Molecular Weight
    
    887.44
    
    Appearance
    
    Solution
    
    Purity
    
    Purity ≥90% by AX-HPLC
    
    Solubility
    
    ≥100.7 mg/mL in H2O; ≥46.3 mg/mL in EtOH; ≥95.6 mg/mL in DMSO
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    O=C1N[[email protected]]2([H])[[email protected]]([[email protected]](CCCCC(NCCCCCC(NC/C=C/C3=CN(C(NC3N)=O)C4OC(COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)C(C4)O)=O)=O)SC2)([H])N1.[Li+].[Li+].[Li+].[Li+]
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Biotin-11-dUTP

Background

Documents

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