Biotin-11-dCTP

- Overview
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    Background
    
    Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.
    Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP
    Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
- Properties
  - Alternative Name
    
    Biotin-X-5-Propargylamino-dCTP; γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
    
    CAS Number
    
    136632-30-9
    
    Molecular Formula
    
    C28H44N7O16P3S (free acid)
    
    Molecular Weight
    
    859.67 (free acid)
    
    Appearance
    
    Solution
    
    Purity
    
    98.00%
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    O=C1N([[email protected]@H]2O[[email protected]](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[[email protected]@H](O)C2)C=C(C#CCNC(CCCCCNC(CCCC[[email protected]@]3([H])[[email protected]](NC(N4)=O)([H])[[email protected]]4([H])CS3)=O)=O)C(N)=N1
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Biotin-11-dCTP

Background

Documents

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