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Figure 1. Immunohistochemical identification of Bombesin Receptor 2/Gastrin-Relasing Peptide Receptor (BB2/GRPR) in human breast carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-BB2/GRPR (Bombesin Receptor 2/Gastrin-Relasing Peptide Receptor) antibody (7TM0039N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, BB2/GRPR receptors were detected at the plasma membrane of nearly all cells in human breast carcinoma.

Figure 2. Immunohistochemical identification of Bombesin Receptor 2/Gastrin-Relasing Peptide Receptor (BB2/GRPR) in human breast carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-BB2/GRPR (Bombesin Receptor 2/Gastrin-Relasing Peptide Receptor) antibody (7TM0039N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, BB2/GRPR receptors were detected at the plasma membrane of nearly all cells in human breast carcinoma.

Figure 3. Validation of the Bombesin Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Bombesin Receptor 2 (BB2) were lysed and immunoblotted with the anti-BB2 antibody (7TM0039N) at a dilution of 1:1000.

BB2 ( IHC-grade ), Bombesin Receptor 2/Gastrin-Releasing Peptide Receptor Polyclonal Antibody

BB2 ( IHC-grade ), Bombesin Receptor 2/Gastrin-Releasing Peptide Receptor Polyclonal Antibody

Catalog Number:

A001455997TMA

Mfr. No.:

7TM0039N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The BB2/GRPR receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Bombesin Receptor 2 (previously called Gastrin-Releasing Peptide Receptor). It can be used to detect total BB2 receptors in Western blots independent of phosphorylation. The BB2 antibody can also be used to isolate and enrich BB2 receptors from cell and tissue lysates. It also detects BB2/GRPR in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide with sequence TNPSVATFSLINGNICHERYV corrsponds to amino acids 364-384 of human BB2
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  BB2, GRPR, Bombesin Receptor 2, Gastrin-releasing Peptide Receptor (UniProt:P30550)
  
  * This product is intended for research use only.

Applications

Application

WB; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Röcken C, Schulz S. Immunohistochemical detection of bombesin receptor subtypes GRP-R and BRS-3 in human tumors using novel antipeptide antibodies. Virchows Arch. 2006 Oct;449(4):421-7. Epub 2006 Sep 12. PubMed PMID: 16967266.

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