CXCR4 ( IHC-grade ), CXC Chemokine Receptor 4 Polyclonal Antibody

Overview
- The CXCR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human CXCR4. It can be used to detect CXCR4 receptors in Western blots in a phosphorylation-sensitive manner. After agonist activation CXCR4 is phosphorylated at S346/S347. Because the CXCR4 antibody detects the same epitope, it no longer binds to the receptor. It detects selectively CXCR4 receptors that have been hot activated and not phosphorylated. Total CXCR4 can be detected in Western blots and immunohistochemistry only after phosphatase treatment. It can also be used to isolate and enrich CXCR4 receptors from brain lysates. It also detects CXCR4 in cultured cells and tissue sections by immunohistochemistry. The CXCR4 antibody has been validated using knockout mice (KO-Validated).
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic peptide with the sequence KGKRGGHSSVSTESESSSFHSS corresponding to residues 338-359 in human, mouse and rat CXCR4.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  CXCR4, CXC Chemokin Receptor 4 (UniProt:P61073 (human), P70658 (mouse), O08565 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Höllt V, Schulz S. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci. 2002 Jul 15;22(14):5865-78.
  Stumm RK, Zhou C, Ara T, Lazarini F, Dubois-Dalcq M, Nagasawa T, Höllt V, Schulz S. CXCR4 regulates interneuron migration in the developing neocortex. J Neurosci. 2003 Jun 15;23(12):5123-30.
  Fischer T, Nagel F, Jacobs S, Stumm R, Schulz S. Reassessment of CXCR4 chemokine receptor expression in human normal and neoplastic tissues using the novel rabbit monoclonal antibody UMB-2. PLoS One. 2008;3(12):e4069. doi: 10.1371/journal.pone.0004069. Epub 2008 Dec 31.
  Sánchez-Alcañiz JA, Haege S, Mueller W, Pla R, Mackay F, Schulz S, López-Bendito G, Stumm R, Marín O. Cxcr7 controls neuronal migration by regulating chemokine responsiveness. Neuron. 2011 Jan 13;69(1):77-90. doi: 10.1016/j.neuron.2010.12.006.
  Gourni E, Demmer O, Schottelius M, D'Alessandria C, Schulz S, Dijkgraaf I, Schumacher U, Schwaiger M, Kessler H, Wester HJ. PET of CXCR4 expression by a (68)Ga-labeled highly specific targeted contrast agent. J Nucl Med. 2011 Nov;52(11):1803-10. doi: 10.2967/jnumed.111.098798.
  Otte M, Kliewer A, Schütz D, Reimann C, Schulz S, Stumm R. CXCL14 is no direct modulator of CXCR4. FEBS Lett. 2014 Dec 20;588(24):4769-75. doi: 10.1016/j.febslet.2014.11.009. Epub 2014 Nov 18.
  Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

CXCR4 ( IHC-grade ), CXC Chemokine Receptor 4 Polyclonal Antibody

CXCR4 ( IHC-grade ), CXC Chemokine Receptor 4 Polyclonal Antibody

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