ARF5 Recombinant Protein

ARF5 Recombinant Protein

Catalog Number:
P001415984ABE
Mfr. No.:
Abe32-3225
Price:
  • Size:
    20 µg
    Quantity:
    Add to Cart:
      • Overview
        • ARF5 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids (1-180 a.a) and having a molecular mass of 22.6kDa. ARF5 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ADP-ribosylation factor 5 (ARF5) is a small guanine nucleotide-binding protein which enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the connection of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation.

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      • Properties
        • Protein Name
          ADP-ribosylation factor 5, ARF5.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 90.0% as determined by SDS-PAGE.
          Formulation
          ARF5 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 0.1M NaCl.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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