APRT cDNA

- Overview
  - APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway
    
    PRODUCT INFORMATION
    Antigen Species: Human
    NCBI Accession No: NP_000476.1
    RNA Reference Number: NM_000485.2
    OMIM Number: 102600
    Chromosome Location: 16q24
    cDNA size: 543bp
    Vector description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
    
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- Properties
  - Protein Name
    
    AMP, APRTD
    
    Type
    
    cDNAs
    
    Formulation
    
    Lyophilized
    
    Storage
    
    Store the plasmid at -20°C.
    
    Sequence
    
    Nucleotide Sequence: ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG TGA
    Translation Sequence: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    (1) Centrifuge at 7000rpm for 1 minute. (2) Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.

APRT cDNA

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