GALT cDNA

- Overview
  - GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene
    
    PRODUCT INFORMATION
    Antigen Species: Human
    NCBI Accession No: NP_000146.2
    RNA Reference Number: NM_000155.3
    OMIM Number: 606999
    Chromosome Location: 9q13
    cDNA size: 1140bp
    Vector description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
    
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- Properties
  - Type
    
    cDNAs
    
    Formulation
    
    Lyophilized
    
    Storage
    
    Store the plasmid at -20°C.
    
    Sequence
    
    Nucleotide Sequence: ATGTCGCGCA GTGGAACCGA TCCTCAGCAA CGCCAGCAGG CGTCAGAGGC GGACGCCGCA GCAGCAACCT TCCGGGCAAA CGACCATCAG CATATCCGCT ACAACCCGCT GCAGGATGAG TGGGTGCTGG TGTCAGCTCA CCGCATGAAG CGGCCCTGGC AGGGTCAAGT GGAGCCCCAG CTTCTGAAGA CAGTGCCCCG CCATGACCCT CTCAACCCTC TGTGTCCTGG GGCCATCCGA GCCAACGGAG AGGTGAATCC CCAGTACGAT AGCACCTTCC TGTTTGACAA CGACTTCCCA GCTCTGCAGC CTGATGCCCC CAGTCCAGGA CCCAGTGATC ATCCCCTTTT CCAAGCAAAG TCTGCTCGAG GAGTCTGTAA GGTCATGTGC TTCCACCCCT GGTCGGATGT AACGCTGCCA CTCATGTCGG TCCCTGAGAT CCGGGCTGTT GTTGATGCAT GGGCCTCAGT CACAGAGGAG CTGGGTGCCC AGTACCCTTG GGTGCAGATC TTTGAAAACA AAGGTGCCAT GATGGGCTGT TCTAACCCCC ACCCCCACTG CCAGGTATGG GCCAGCAGTT TCCTGCCAGA TATTGCCCAG CGTGAGGAGC GATCTCAGCA GGCCTATAAG AGTCAGCATG GAGAGCCCCT GCTAATGGAG TACAGCCGCC AGGAGCTACT CAGGAAGGAA CGTCTGGTCC TAACCAGTGA GCACTGGTTA GTACTGGTCC CCTTCTGGGC AACATGGCCC TACCAGACAC TGCTGCTGCC CCGTCGGCAT GTGCGGCGGC TACCTGAGCT GACCCCTGCT GAGCGTGATG ATCTAGCCTC CATCATGAAG AAGCTCTTGA CCAAGTATGA CAACCTCTTT GAGACGTCCT TTCCCTACTC CATGGGCTGG CATGGGGCTC CCACAGGATC AGAGGCTGGG GCCAACTGGA ACCATTGGCA GCTGCACGCT CATTACTACC CTCCGCTCCT GCGCTCTGCC ACTGTCCGGA AATTCATGGT TGGCTACGAA ATGCTTGCTC AGGCTCAGAG GGACCTCACC CCTGAGCAGG CTGCAGAGAG ACTAAGGGCA CTTCCTGAGG TTCATTACCA CCTGGGGCAG AAGGACAGGG AGACAGCAAC CATCGCCTGA
    Translation Sequence: MSRSGTDPQQ RQQASEADAA AATFRANDHQ HIRYNPLQDE WVLVSAHRMK RPWQGQVEPQ LLKTVPRHDP LNPLCPGAIR ANGEVNPQYD STFLFDNDFP ALQPDAPSPG PSDHPLFQAK SARGVCKVMC FHPWSDVTLP LMSVPEIRAV VDAWASVTEE LGAQYPWVQI FENKGAMMGC SNPHPHCQVW ASSFLPDIAQ REERSQQAYK SQHGEPLLME YSRQELLRKE RLVLTSEHWL VLVPFWATWP YQTLLLPRRH VRRLPELTPA ERDDLASIMK KLLTKYDNLF ETSFPYSMGW HGAPTGSEAG ANWNHWQLHA HYYPPLLRSA TVRKFMVGYE MLAQAQRDLT PEQAAERLRA LPEVHYHLGQ KDRETATIA.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    (1) Centrifuge at 7000rpm for 1 minute. (2) Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.

GALT cDNA

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